The present study revealed a weak antiplasmodial activity of both aqueous and methanol extracts of the plant. To date, there are no efficacy or safety studies carried out to support its ethnomedicinal use in malaria management. Ellagic acid and the new compound 3,4,5trimethoxyphenyl6ogalloylo. Antiplasmodial activity of leaf extracts of zanthoxylum chalybeum engl.
Jan 28, 2016 resistance development in human malaria parasites against commonly used antimalarial drugs has necessitated the scientific exploration of traditionally used antimalarial plants. Screening of plant extracts for searching antiplasmodial activity. Antiplasmodium falciparum activity of extracts from 10. In vivo and in vitro antimalarial activity of vernonia. Noumedem anangmo christelle nadia 1, wabo pone j 1, kumar kaushi n 2, mohana krishnan d 2, mittal garima 2, nguemfo tchakugni arlette 1, mishral shubbi 2, mpoame mbida 1 and dinkar sahal 2. In vitro antiplasmodial and cytotoxic activity of norcaesalpin d and extracts of four plants. Details of medicinal plants tested for antiplasmodial activity and. In vitro antiplasmodial activity of crude extracts of. In order to assess their acclaimed potentials, eleven extracts were prepared from seven selected plants commonly used in western cameroon, and tested both for their antiplasmodial activity and cytotoxicity. In vitro and in vivo antiplasmodial activity of extracts from polyalthia suaveolens, uvaria angolensis and monodora tenuifolia annonaceae alvine n.
This study provides the first direct evidence of the antichaperone function and antiplasmodial activity of extracts from both z. International journal of medicinal plant research, 1 5. The method employed for obtaining the extracts was silica gel column chromatography, and the techniques used for the analysis of antiplasmodial activity ic 50. In vitro antiplasmodial activity of some medicinal plants. Invivo antiplasmodial activity of methanol whole plant. The antitrypanosomal activity of 101 crude ethanol extracts derived from 88 medicinal plants from cote divoire was determined in vitro using trypanosoma brucei rhodesiense. Flavonoid versus artemisinin antimalarial activity in. Flavonoids had no effect on the in vitro antiplasmodial activity of a.
Antiplasmodial potential of medicinal plant extracts from malaiyur and javadhu hills of south india. Invivo antiplasmodial activity of methanol whole plant extracts of tapinanthus dodoneifolius dc danser in mice z. Antimicrobial activity of crude extracts of three medicinal. The following antibiotic solutions were prepared prior incorporation into the liquid medium. At lower concentrations, trophozoites and schizones persisted with defects in morphological conditions. The selected plants, acokanthera oppositifolia lam apocynaceae is a. Evaluation of in vivo antiplasmodial activities of extracts. The chloroform extract of the leaves was further bioassayguided fractionated using a combination of centrifugal partition. The aim of the study was to evaluate the in vitro antiplasmodial. Antiplasmodial activity and phytochemical analysis of extracts from selected ugandan medicinal plants. However, the presence of the alkyl group in the ir data could signify the presence of terpenoids which is consistent with the potent antiplasmodial activity that it exhibited. In vitro antiplasmodial activity of flower extracts from. The presence of the alkaloids in the extract could then explain the moderate antiplasmodial activity observed. In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of phyllanthus niruri l.
In vitro antiplasmodial activity of medicinal plants native to or naturalised in south africa. Nov 23, 2011 malaria remains a major public health problem, especially in tropical and subtropical regions because of the emergence and widespread of antimalarial drug resistance. Datasheet of 221 plant extracts displaying variation in antiplasmodial activity was prepared. Sep 12, 2017 abdullahi z, anuka ja, salawu ao, hussaini im 2015 invivo antiplasmodial activity of methanol whole plant extracts of tapinanthus dodoneifolius dc danser in mice.
In vitro and in vivo antiplasmodial activity of extracts from polyalthia suaveolens, uvaria angolensis and. Various extracts obtained from 19 plants collected in kenya were tested for antiplasmodial activity. In vitro hemolysis of human erythrocytes by plant extracts. Invivo antiplasmodial activity of crude nhexane and. The in vitro antiplasmodial activity of the plant extracts was evaluated by isotopic microtest, while the in vivo antimalarial activity was determined by the classical 4day suppressive test. Three plant extracts caused 100% inhibition of the parasite growth at concentrations of plant material. The ic50s, as determined by parasite dna concentration, ranged from 200 and 200. The antiplasmodial activities of the plant extracts when administered twice daily were not significantly different p 0. The use of microfluorometric method for activityguided. Antiplasmodial activity of methanolic leaf extract of. The most promising fraction was subjected to column. The antiplasmodial activity was assessed using lactate dehydrogenase assay pldh and the cytotoxicity estimated on llcmk2 monkey kidney. Our findings suggest that the two plants contain compounds that inhibit parasite growth in vitro.
The antiplasmodial activity of dichloromethane and methanolic extracts was tested on p. Abdullahi z, anuka ja, salawu ao, hussaini im 2015 invivo antiplasmodial activity of methanol whole plant extracts of tapinanthus dodoneifolius dc danser in mice. From the studies conducted on the plant until now, it is known that the methanolic root extract of the plant has schizonticidal activity as high as 84. Antiplasmodial activity of the ethnobotanical plant cassia. Tml and tmr showed in vitro antiplasmodial activity against fcb1 strain of p. The present study revealed a weak antiplasmodial activity of both aqueous and methanol extracts of. Potent antiplasmodial extracts and fractions from terminalia. Screening of plant extracts for searching antiplasmodial. Pdf in vitro antiplasmodial activity of medicinal plants. Antiplasmodial activity was observed from mice blood treated by various concentration of either extract or isolate and the parasitaemia percentage were determined by calculating infected blood cell after 24 h of the treatment.
Malaria journal evaluation of antiplasmodial activity of medicinal plants from north indian buchpora and south indian eastern ghats naveen k kaushik 1 2 asokan bagavan 0 abdul a rahuman 0 abdul a zahir 0 chinnaperumal kamaraj 0 gandhi elango 0 chidambaram jayaseelan 0 arivarasan v kirthi 0 thirunavukkarasu santhoshkumar 0 sampath marimuthu 0 govindasamy rajakumar 0 santosh k. In a screen of 1800 plant and fungal extracts for antiplasmodial, antitrypanosomal, and leishmanicidal activity, the nhexane extract of chrysanthemum cinerariifolium trevir. Plant derivatives have been used for curing malaria historically. Here, we investigated the in vitro antiplasmodial activity of bark extracts from two fabaceae species tetrapleura tertaptera and copaifera religiosa. All plant samples displayed various antiplasmodial activity. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Aqueous preparations of the whole plant of tapinanthus dodoneifolius dc danser growing on parkia biglobosa tree are used by the nomads in northern part of nigeria for managing malaria, and many other ailments example, diabetes, fever, diarrhoea and wounds. The present study aimed at investigating the in vitro and in vivo susceptibility of malaria parasites to crude extracts and fractions from polyalthia suaveolens, uvaria angolensis, and monodora tenuifolia. Antiplasmodial activity and toxicity of crude extracts. In vitro and in vivo antiplasmodial activity of extracts from. In vitro antiplasmodial activity and cytotoxicity of extracts. Evaluation of the antiplasmodial activity of extracts of. In vitro antiplasmodial activity of extracts of tristaniopsis. Six extracts exhibited good activity against both pf3d7 and pfindo strains, including cold water, water decoction, and ethyl acetate extracts of leaves of drypetes principum mull.
In vivo validation of antimalarial activity of crude. Antiplasmodial activity an overview sciencedirect topics. The results showed that all extracts were active with ic 50 0. This study revealed for the first time the antiplasmodial activity. The chemical examination follows after the isolation and biological screening of the active fraction1. In vitro antiplasmodial activity and cytotoxicity of. The ethanolic extracts were prepared by maceration, and were further partitioned using water, dichloromethane, hexane, and methanol. Studies were carried out to determine the antiplasmodial activity of crude nhexane and ethanolic seed extracts of moringa oleifera using cold extraction method. In vitro antiplasmodial activity of crude extracts from. Of those extracts 8 showed good activity ic 50 values. These values reflect a good antiplasmodial activity in vitro, assuming that ic50 of the potential sources of antimalarial drugs is traditional medicinal plants. Abdullahi department of pharmacology and toxicology, kaduna state university, kadunanigeria. Phytochemical investigation and biological activity of. For extracts of cassia occidentalis leaves, morinda morindoides leaves and phyllanthus niruri whole plant, the in vivo antiplasmodial activity has already been reported tona et al.
Antiplasmodial and antitrypanosomal activity of pyrethrins. In the present work, the antiplasmodial activities of five extracts crude, hexane, chloroform, ethyl acetate and methanol from the flowers of combretum leprosum are assessed. Eleven crude extracts prepared from seven different plant species from western cameroon were screened in vitro for their antiplasmodial activity and cytotoxicity e. Flavonoids have been suggested as candidates for increasing antiplasmodial activity and potentially slowing the emergence of resistance in wholeplant preparations, relative to artemisinin alone weathers et al. Evaluation of antiplasmodial activity of medicinal plants. The selected plants, acokanthera oppositifolia lam apocynaceae is a species encountered in eastern. Nov 11, 2007 extracts were subjected to in vitro antiplasmodial screening against wild strains of plasmodium falciparum using the nitro. Jan 24, 2008 in vitro antiplasmodial activity of methanolic extracts of 16 medicinal plants was evaluated by fluorometric assay using picogreen.
One of the potential sources of antimalarial drugs is traditional medicinal plants. Thus, the present study aims at evaluating the invivo antiplasmodial activity of solvent fractions of methanolic root extract of dodonaea angustifolia. The antioxidant activity of the plant extracts using the. Plant extracts were categorized into different classes on the basis of their antiplasmodial activity. Host cell toxicity was analyzed on mrc5 human diploid embryonic lung cells using the mtt test.
Four crude extracts showing promising antiplasmodial activity profile were selected and fractionated via successive solidliquid extractions using three different organic solvents n. Thus, the present study aims at evaluating the invivo antiplasmodial activity of solvent fractions of methanolic root extract of. It is expressed as decreased of parasitaemia levels and percent of inhibition. Propagation in culture of plasmodium falciparum gametocytes. Materials and methods collection of plant materials jatropha tanjorensis leaves were collected during the. In vitro antiplasmodial activity of methanolic extracts of 16 medicinal plants was evaluated by fluorometric assay using picogreen. Microwave assisted extraction mae has been used to increase biological activities, to reduce. Antiplasmodial activity of solvent fractions of methanolic. Malaria remains a major public health problem, especially in tropical and subtropical regions because of the emergence and widespread of antimalarial drug resistance. Antiplasmodial activity of norcaesalpin d and extracts of. Antiplasmodial activity of ethanolic extract of macaranga. Antibacterial activity of plant extracts 249 culture media, antibiotics and phytochemical solutions brain heart infusion as liquid and solid media and muellerhinton agar were used. Preliminary in vitro antiplasmodial activity of aristolochia. The aim objective of this study consists in isolating the active molecules from plants used in traditional medicine to treat malaria and its symptoms.
Four crude extracts showing promising antiplasmodial activity profile were selected and fractionated via successive solidliquid extractions using three different organic solvents nhexane, chloroform, ethyl acetate. Traditional medicine represents one potential source of new treatments. Malaria journal evaluation of antiplasmodial activity of medicinal plants from north indian buchpora and south indian eastern ghats naveen k kaushik 1 2 asokan bagavan 0 abdul a rahuman 0 abdul a zahir 0 chinnaperumal kamaraj 0 gandhi elango 0 chidambaram jayaseelan 0 arivarasan v kirthi 0 thirunavukkarasu santhoshkumar 0 sampath marimuthu 0 govindasamy rajakumar 0. Antitrypanosomal and antiplasmodial activity of medicinal. Of the 16 extracts tested, 9 showed significant antiplasmodial activity at concentrations less than 50.
Twentyfour albino mice mus musculus induced intraperitoneally with chloroquine sensitive plasmodium berghei strain were divided into 4 groups and treated at three concentrations viz. Several members of the fabaceae family are reported to have antiplasmodial activity 4, 5, 6, 7. In this study we have attempted to assess the rate of hemolysis with different plant extracts and their fractions. Ten plants indigenous to sudan and of common use in sudanese folkmedicine, were examined in vitro for antimalarial activity against schizonts maturation of plasmodium falciparum, the major human malaria parasite. Antiplasmodial activity can also be identified by the life cycle inhibition of plasmodium.
Preliminary phytochemical analysis and in vivo antimalarial. A good correlation between the in vitro and in vivo antiplasmodial activity of these selected medicinal plant species exists. Antiplasmodial activity of norcaesalpin d and extracts of e. Screening of plants from new caledonia for antiplasmodial activity against plasmodium falciparum revealed that methanolic extracts of the leaves and bark of tristaniopsis calobuxus, t. Tetrapleura tetraptera and copaifera religiosa are perennial trees widely distributed in gabon. In vitro antiplasmodial activity of extracts and fractions. In this work, we studied the in vitro antiplasmodial activity of chloromethylenic, methanolic, and meohh2o 11 crude extracts and decoction obtained from eight medicinal plants collected in burkina faso and of total alkaloids for five plants. Antiplasmodial activity and cytotoxicity of fractions from the most promising extracts.
Pdf antiplasmodial activity and phytochemical analysis of. A mean survival time of 19 days observed in the root extract supported its antiplasmodial activities compared with other plant parts. Read antiplasmodial activity and toxicity of crude extracts from alternatives parts of plants widely used for the treatment of malaria in burkina faso. Antiplasmodial activity of some medicinal plants used in. In this work, we studied the in vitro antiplasmodial activity of chloromethylenic, methanolic, and meohh2o 11 crude extracts and decoction obtained from eight medicinal plants collected in burkina faso and of. Read antiplasmodial activity of extracts and quassinoids isolated from seedlings of ailanthus altissima simaroubaceae, phytotherapy research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Crude extracts from leaves, bark and fruits were tested for their antiplasmodial activity against the chloroquinesensitive strain of plasmodium falciparumd10, where leaf extracts showed the highest activity. Pdf antiplasmodial potential of medicinal plant extracts. Fifteen extracts from nine selected argentine medicinal plants were tested for their antiplasmodial activity in vitro by assessing their ability to inhibit the uptake of 3hhypoxanthine into the.
Methods plants and chemicals aristolochia griffithii hook, commonly called nagbal. The present study involves in vitro evaluation of two medicinally important plants aristolochia griffithii and thalictrum foliolosum dc used in. Antiplasmodial activity of plant extracts used in west. However, if a previous study revealed that bauhinia rufescens is used in some recipes against malaria, this in vitro study demonstrated that there is no significant antiplasmodial activity in any extract of this plant. The antiplasmodial activities of the ether and methanol extracts were expressed as 50% effective. Medicinal plants play a key role in malaria control in africa, especially in remote areas where health facilities are limited. The antiplasmodial activity 50% inhibitory concentration, ic50 of plant extracts ranged from 1. In vivo antiplasmodial activity of two sahelian plant. The two most active extracts that produced 100% inhibition of the parasite growth at concentration of plant material. Resistance development in human malaria parasites against commonly used antimalarial drugs has necessitated the scientific exploration of traditionally used antimalarial plants.
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